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    ASIA unversity > 醫學暨健康學院 > 期刊論文 >  Item 310904400/6596


    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/6596


    Title: Mutation analysis of Taiwanese Wilson disease patients
    Authors: Lei Wan;Chang-Hai Tsai;Yuhsin Tsai;Hsu CM;Lee,Chun-Cheng;Tsai,Fuu-Jen
    Date: 2006-06
    Issue Date: 2009-12-23 14:20:51 (UTC+8)
    Publisher: Asia University
    Abstract: Wilson disease (WD) is an autosomal recessive disorder of copper metabolism, which is caused by mutation in copper-transporting ATPase (ATP7B). In the present study, we report a molecular diagnosis method to screen the WD chromosome in patients or in heterozygotic carriers in Taiwan. Exons 8, 11, 12, 13, 16, 17, and 18 of ATP7B are selected for the screening of mutations. The most common mutation, Arg778Leu or Arg778Gln, was first screened by PCR-RFLP then we combined single-stranded conformation polymorphism (SSCP) analysis followed by direct DNA sequencing on the DNA fragments with mobility shift on SSCP analysis. The diagnostic rate was compared with standard ATP7B whole gene sequencing analysis. Ten different mutations were identified among 29 WD patients; among them four were novel (Ala1168Pro, Thr1178Ala, Ala1193Pro, and Pro1273Gln). The false positive rates were tested against 100 normal individuals and listed as follows: exon 8: 5%; exon 11: 4%; exon 12: 6%; exon 13: 5%; exon 16: 5%; exon 17: 3%; exon 18: 4%. The Arg778Leu mutation exhibited the highest allelic frequency (43.1%). The detection rate of WD chromosomes is 65.52%, which is as sensitive as whole gene sequencing scanning. According to our results, WD chromosomes in Taiwan are predominantely located at exons 8, 11, 12, 13, 16, 17, and 18. The standard sequencing analysis on the entire gene is time consuming. We recommend screening these 7 exons first on those individuals who have a higher risk in having WD, before whole gene and promoter sequencing analysis in Taiwan.
    Relation: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (345): 734-738
    Appears in Collections:[醫學暨健康學院] 期刊論文

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