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    Title: Anti-SARS coronavirus 3C-like protease effects of Isatis indifotica root and plant-derived phenolic compounds
    Authors: Cheng-Wen Lin;Tsai,Fuu-Jen;Chang-Hai Tsai;Chien-Chen Lai;Lei Wan;Tin-Yun Ho
    Date: 2005-10
    Issue Date: 2009-12-23 14:21:32 (UTC+8)
    Publisher: Asia University
    Abstract: The 3C-like protease (3CLpro) of SARS-coronavirus mediates the proteolytic processing of replicase polypeptides 1a and 1ab into functional proteins, becoming an important target for the drug development. In this study, Isatis indigotica root extract, five major compounds of I. indigotica root, and seven plant-derived phenolic compounds were tested for anti-SARS-CoV 3CLpro effects using cell-free and cell-based cleavage assays. Cleavage assays with the 3CLpro demonstrated that IC50 values were in micromolar ranges for I. indigotica root extract, indigo, sinigrin, aloe emodin and hesperetin. Sinigrin (IC50: 217 M) was more efficient in blocking the cleavage processing of the 3CLpro than indigo (IC50: 752 M) and beta-sitosterol (IC50: 1210 M) in the cell-based assay. Only two phenolic compounds aloe emodin and hesperetin
    dose-dependently inhibited cleavage activity of the 3CLpro, in which the IC50 was 366 M for aloe emodin and 8.3 M for hesperetin in the cell-based assay.
    Relation: ANTIVIRAL RESEARCH 68 (1): 36-42
    Appears in Collections:[College of Medical and Health Science] Journal Article

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