ASIA unversity:Item 310904400/6790
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    ASIA unversity > 醫學暨健康學院 > 期刊論文 >  Item 310904400/6790


    题名: Karyotypic evolution of a novel cervid satellite DNA family isolated by microdissection from the Indian muntjac Y-chromosome
    作者: Y.-C. Li;Y.-M. Cheng;L.-J. Hsieh;O. A. Ryder;F.Yang;S.-J. Liao;Kuang-Ming Hsiao;Fuu-Jen Tsai;Chang-Hai Tsai;C. C. Lin
    日期: 2005-04
    上传时间: 2009-12-23 14:22:23 (UTC+8)
    出版者: Asia University
    摘要: A minilibrary was constructed from DOP-PCR products using microdissected Y-chromosomes of Indian muntjac as DNA templates. Two microclones designated as IM-Y4-52 and IM-Y5-7 were obtained from negative screening of all three cervid satellite DNAs (satellites I, II, and IV). These two microclones were 295 and 382 bp in size, respectively, and shared 70% sequence homology. Southern blot analysis showed that the IM-Y4-52 clone was repetitive in nature with an 0.32-kb register in HaeIII digest. Sequence comparison revealed no similarities to DNA sequences deposited in the GenBank database, suggesting that the microclone sequences were from a novel satellite DNA family designated as cervid satellite V. A subclone of an Indian muntjac BAC clone which screened positive for IM-Y4-52 had a 3,325-bp insert containing six intact monomers, four deleted monomers, and two partial monomers. The consensus sequence of the monomer was 328 bp in length and shared more than 80% sequence homology with every intact monomer. A zoo blot study using IM-Y4-52 as a probe showed that the strong hybridization with EcoRI digested male genomic DNA of Indian muntjac, Formosan muntjac, Chinese muntjac, sambar deer, and Chinese water deer. Female genomic DNA of Indian muntjac, Chinese water deer, and Formosan muntjac also showed positive hybridization patterns. Satellite V was found to specifically localize to the Y heterochromatin region of the muntjacs, sambar deer, and Chinese water deer and to chromosome 3 of Indian muntjac and the X-chromosome of Chinese water deer.
    關聯: CHROMOSOMA (114): 28-38
    显示于类别:[醫學暨健康學院] 期刊論文


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