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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/7845


    Title: Rapid PCR-RFLP method for identification of five billfish species
    Authors: Hung-Sheng Hsieh;Tuu-Jyi Chai;Deng-Fwu Hwang
    Keywords: PCR-RFLP;billfish;mtDNA;cytochrome b gene;identification
    Date: 2005
    Issue Date: 2010-02-26
    Publisher: Asia University
    Abstract: A rapid and reliable polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to identify billfish species Xiphias gladius (swordfish), Makaira nigricans (blue marlin), Makaira indica (black marlin), Istiophorus platypterus (sailfish), and Tetrapturus audax (striped marlin). After DNA extraction and amplifying, the 348-bp PCR products from gene encoding of cytochrome b were subjected to restriction enzyme analysis. No single enzyme tested was able to distinguish the 5 species at the same time, but the combination of results obtained from the digests of Bsa JI and Cac 8I could be used to differentiate the 5 billfish species. This sensitive, rapid, and valid method can be used to detect fraudulent substitutes.
    Relation: Journal of Food Science, 70(4):C246-C249.
    Appears in Collections:[食品營養與保健生技學系] 期刊論文

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