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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/79191


    Title: APPLICATION OF LC-MS/MS IN IDENTIFICATION OF TOXIN IN THE CAUSATIVE GASTROPOD AND VICTIM SAMPLES
    Authors: 謝承紘;Hsieh, Cheng-Hong
    Contributors: 保健營養生技學系
    Date: 201312
    Issue Date: 2014-01-06 20:20:23 (UTC+8)
    Abstract: Food paralytic poisoning incident was detected in the gastropod Nassariidae Niotha clathrata and Zeuxis scalaris occurred in December 2012 in Kaohsiung, Taiwan. The toxin was purified from each remain of cooked gastropod and victim tissues by ultrafiltration using a YM-3 membrane, followed by C18 cartridge column purification. Based on analyses by LC-MS/MS, the causative agent of gastropod food poisoning was identified as tetrodotoxin. The toxicity of remain of cooked sample was 245 MU/g (1MU = 0.178 μg TTX) for N. clathrata and 203 MU/g for Z. scalaris. The blood and excrements of victim was shown to contain TTX of 0.13 and 0.18 μg/g, respectively. There was positive between results from LC-MS/MS and mouse bioassay in the causative gastropod. The LC-MS/MS method is suitable for rapid screening of TTX.
    Relation: Journal of Marine Science and Technology-Taiwan; 21:52-57
    Appears in Collections:[食品營養與保健生技學系] 期刊論文

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