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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/79500

    Title: The anti-tumor activity of Antrodia salmonea in human promyelocytic leukemia;HL-60) cells is mediated via the induction of G1 cell-cycle arrest and apoptosis in vitro or in vivo
    Authors: 許游章;Hseu, You-Cheng;李傳珍;Lee, Chuan-Chen;Yung-Chang, C;Chen, Yung-Chang;K.J.Senthil;Kumar, K.J.Senthil;Ch, Chee-Shan;Chen, Chee-Shan;Huang, Yu-Chi;Huang, Yu-Chi;Hsu, Li-Sung;Hsu, Li-Sung;Huan, Hui-Chi;Huang, Hui-Chi;Ya, Hsin-Ling;*, Hsin-Ling Yang
    Contributors: 保健營養生技學系
    Keywords: Antrodia salmonea;Human promyelocytic leukemia;G1 cell-cycle arrest;Apoptosis;Xenografted nude mice
    Date: 2014-04
    Issue Date: 2014-06-03 19:22:57 (UTC+8)
    Abstract: Ethnopharmacological relevance
    The medicinal mushroom Antrodia salmonea has been used as a traditional Chinese medicine and has demonstrated antioxidant and anti-inflammatory effects.

    Materials and methods
    In the present study, we examined the anti-tumor activity of the fermented culture broth of Antrodia salmonea (AS) in vitro and in vivo and revealed its underlying molecular mechanism of action.

    Treatment of human promyelocytic leukemia (HL-60) cells with AS (50–150 μg/mL) significantly reduced cell viability and caused G1 arrest via the inhibition of cell-cycle regulatory proteins, including cyclin D1, CDK4, cyclin E, cyclin A, and phosphorylated retinoblastoma protein (p-Rb). Furthermore, AS treatment induced apoptosis, which was associated with DNA fragmentation, followed by a sequence of events, including intracellular ROS generation; mitochondrial dysfunction; Fas ligand activation; cytochrome c release; caspase-3, -8, -9, and PARP activation; and Bcl-2/Bax dysregulation. The results of the in vitro study suggested that AS-induced apoptosis in HL-60 cells was mediated by both the mitochondrial and death receptor pathways. Furthermore, we found that AS treatment was effective in delaying tumor incidence in HL-60 xenografted nude mice and reducing tumor burden.

    To the best of our knowledge, this is the first report confirming the anti-tumor activity of this potentially beneficial mushroom against human promyelocytic leukemia.
    Relation: JOURNAL OF ETHNOPHARMACOLOGY,153 (2014),499–510.
    Appears in Collections:[食品營養與保健生技學系] 期刊論文

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