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|Title: ||The anti-tumor activity of Antrodia salmonea in human promyelocytic leukemia;HL-60) cells is mediated via the induction of G1 cell-cycle arrest and apoptosis in vitro or in vivo|
|Authors: ||許游章;Hseu, You-Cheng;李傳珍;Lee, Chuan-Chen;Yung-Chang, C;Chen, Yung-Chang;K.J.Senthil;Kumar, K.J.Senthil;Ch, Chee-Shan;Chen, Chee-Shan;Huang, Yu-Chi;Huang, Yu-Chi;Hsu, Li-Sung;Hsu, Li-Sung;Huan, Hui-Chi;Huang, Hui-Chi;Ya, Hsin-Ling;*, Hsin-Ling Yang|
|Keywords: ||Antrodia salmonea;Human promyelocytic leukemia;G1 cell-cycle arrest;Apoptosis;Xenografted nude mice|
|Issue Date: ||2014-06-03 19:22:57 (UTC+8)|
|Abstract: ||Ethnopharmacological relevance
The medicinal mushroom Antrodia salmonea has been used as a traditional Chinese medicine and has demonstrated antioxidant and anti-inflammatory effects.
Materials and methods
In the present study, we examined the anti-tumor activity of the fermented culture broth of Antrodia salmonea (AS) in vitro and in vivo and revealed its underlying molecular mechanism of action.
Treatment of human promyelocytic leukemia (HL-60) cells with AS (50–150 μg/mL) significantly reduced cell viability and caused G1 arrest via the inhibition of cell-cycle regulatory proteins, including cyclin D1, CDK4, cyclin E, cyclin A, and phosphorylated retinoblastoma protein (p-Rb). Furthermore, AS treatment induced apoptosis, which was associated with DNA fragmentation, followed by a sequence of events, including intracellular ROS generation; mitochondrial dysfunction; Fas ligand activation; cytochrome c release; caspase-3, -8, -9, and PARP activation; and Bcl-2/Bax dysregulation. The results of the in vitro study suggested that AS-induced apoptosis in HL-60 cells was mediated by both the mitochondrial and death receptor pathways. Furthermore, we found that AS treatment was effective in delaying tumor incidence in HL-60 xenografted nude mice and reducing tumor burden.
To the best of our knowledge, this is the first report confirming the anti-tumor activity of this potentially beneficial mushroom against human promyelocytic leukemia.
|Relation: ||JOURNAL OF ETHNOPHARMACOLOGY,153 (2014),499–510.|
|Appears in Collections:||[食品營養與保健生技學系] 期刊論文|
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