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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/79503


    Title: Bufalin-Inhibited Migration and Invasion in Human Osteosarcoma U-2 OS Cells Is Carried Out by Suppression of the Matrix Metalloproteinase-2, ERK, and JNK Signaling Pathways
    Authors: 闕甫伈;Chueh, Fu-Shin;Ya-Yin Chen,;Ya-Yin Chen,;Hua, An-Cheng;Huang, An-Cheng;Heng-Chien, H;Heng-Chien Ho,;Ching-Lung, L;Ching-Lung Liao,;Yan, Jai-Sing;Jai-Sing Yang,;Kuo, Chao-Lin;Chao-Lin Kuo,;鍾景光;*, Jing-Gung Chung
    Contributors: 保健營養生技學系
    Keywords: bufalin;human osteosarcoma U-2 OS cells;matrix metalloproteinases;migration and invasion;traditional Chinese medicine
    Date: 2014-01
    Issue Date: 2014-06-03 19:23:21 (UTC+8)
    Abstract: Bufalin has been shown to exhibit multiple pharmacological activities, including induction of apoptosis in many types of cancer cell lines. Osteosarcoma is a type of cancer which is difficult to treat and the purpose of this study was to investigate the effects of bufalin on the migration and invasion of human osteosarcoma U-2 OS cells. The wound healing assay and Boyden chamber transwell assay were used for examining the migration of U-2 OS cells. Western blotting and gelatin zymography assays were used for theexpression and activities of metalloproteinase (MMP)-2, MMP-7 or MMP-9 levels. Western blotting analysis also was used for measuring the levels of growth factor receptor-bound protein 2 (GRB2), son of sevenless homolog 1 (SOS1), c-Jun N-terminal kinases 1/2 (JNK1/2), extracellular signal-regulated kinase 1/2 (ERK1/2), and p38 in bufalin-treated U-2 OS cells. Bufalin inhibited the cell migration and invasion of U-2 OS cells in vitro. Moreover, bufalin reduced MMP-2 and MMP-9 enzyme activities of U-2 OS cells. Bufalin also suppressed the protein level of MMP-2 and reduced the levels of mitogen-activated protein kinases (MAPKs) such as JNK1/2 and ERK1/2 signals in U-2 OS cells. Our results suggest that signaling pathways for bufalin-inhibited migration and invasion of U-2 OS cells might be mediated through blocking MAPK signaling and resulting in the inhibition of MMP-2. Bufalin could be a useful agent to develop as a novel antitumor agent by virtue of its ability to inhibit tumor cell migration and invasion.
    Copyright © 2011 Wiley Periodicals, Inc.
    Relation: Environmental Toxicology; 29(1):21-9.
    Appears in Collections:[食品營養與保健生技學系] 期刊論文

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