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    题名: Authentication of consumer fraud in Taiwanese fish products by molecular trace evidence and forensically informative nucleotide sequencing
    作者: 謝承紘;Hsieh, Cheng-Hong
    贡献者: 保健營養生技學系
    关键词: Molecular trace evidence;Food authenticity;Consumer fraud;Forensically informative nucleotide sequencing;BLAST;Cytochrome b gene
    日期: 2014-01
    上传时间: 2014-06-03 19:23:46 (UTC+8)
    摘要: An inexpensive, efficient, and reliable molecular method of authenticating commercial puffer fish-based products was developed to determine the origin of adulterated fish products and for food regulatory control. This system is based on molecular trace evidence obtained using a specific primer set with a short gene marker located on the mitochondrial cytochrome b sequence region. The method was successfully tested and validated in 12 specimens of puffer fish, simulated products, and 50 commercial samples. Fourteen percent of the collected commercial products were found to be puffer fish-based. However, of these 14%, 28% were identified as toxic varieties by further analysis with forensically informative nucleotide sequencing (FINS) and BLAST methods. These results reveal that the developed method is a rapid and efficient tool to unequivocally identify puffer fish, which may aid in the prevention of consumer fraud or mislabeling of fish products.
    显示于类别:[食品營養與保健生技學系] 期刊論文


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