Proliferative effects of chishao on Schwann cells are FGF-uPA, and ERK- and JNK-dependent

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/79536

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Title:	Proliferative effects of chishao on Schwann cells are FGF-uPA, and ERK- and JNK-dependent
Authors:	Hua, Kun Shan;Huang, Kun Shan;Lu, Min-Jin;Lu, Min-Jin;陳悅生;Chen, Yueh-Sheng;蔡輔仁;Tsai, Fuu-Jen;黃志揚;HUANG, CHIH-YANG;Che, Yi-Chang;Cheng, Yi-Chang;林建仲;Lin, Chien-Chung;蔡長海
Contributors:	生物科技學系
Keywords:	Chishao;Proliferative Effect;Schwann Cells;Nerve Regeneration
Date:	2014
Issue Date:	2014-06-04 10:13:11 (UTC+8)
Abstract:	This study evaluated the proliferative effects of chishao on RSC96, Schwann cells. A dose-dependent proliferative effect of chishao was obtained by methylthiazol tetrazolium (MTT), proliferating cell nuclear antigen (PCNA) Western blotting, and wound healing assays in Schwann cells administered with chishao (0-500 mg/ml), except at 500 mg/ml concentration. The chishao-treated cells also showed a dose-dependent activated fibroblast growth factor-2 (FGF-2) signaling with increased urokinase plasminogen activator (uPA) and decreased plasminogen activator inhibitor-1 (PAI-1), enhanced proliferative proteins, extracellular signal regulated kinase (ERK) and c-Jun N-terminal kinase (JNK)-signaling. Using mitogen-actvated protein kinase (MAPK)-signaling chemical inhibitors, U0126, SB203580, and SP600125, the proliferative effects of chishao on RSC cells were identified to be ERK- and JNK- signaling dependent. Based on the results, applying appropriate doses of chishao to Schwann cells would be a potential approach for enhancing neuron regeneration.
Relation:	AMERICAN JOURNAL OF CHINESE MEDICINE;37(6):1191-202.
Appears in Collections:	[生物科技學系] 期刊論文

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