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    ASIA unversity > 管理學院 > 國際企業學系 > 期刊論文 >  Item 310904400/79661

    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/79661

    Title: Reduction of TLR4 mRNA stability and protein expressions through inhibiting cytoplasmic translocation of HuR transcription factor by E2 and/or ERα in LPS-treated H9c2 cardiomyoblast cells
    Authors: Ming-Jen Fan;Rosa Huang-Liu;Chia-Yao Shen;Da-Tong Ju;Yueh-Min Lin;Peiying Pai;Pi-Yu Huang;Tsung-Jung Ho;蔡輔仁;Tsai, Fuu-Jen;蔡長海;黃志揚;CHIH-YANG HUANG
    Contributors: 生物科技學系
    Keywords: E2;ERα;HuR;H9c2 cardiomyoblast cells;LPS
    Date: 2014
    Issue Date: 2014-06-05 11:49:25 (UTC+8)
    Abstract: Our previous results have indicated that Akt mediates 17β-estradiol (E2) and/or estrogen receptor α (ERα) to inhibit lipopolysaccharide (LPS)-induced JNK activity, tumor necrosis factor α (TNFα) protein expression, and exhibits cardioprotective effects. Toll-like receptor 4 (TLR4) mRNAs often contain AU-rich elements (AREs) in their 3’-untranslated regions (3’UTR) which have a high affinity for RNA-binding proteins. It is not known whether E2 and ERα affect TLR4 mRNA stability and TLR4 protein expression through regulating the RNA-binding proteins, human antigen R (HuR), tristetraprolin (TTP) and AU-binding factor 1 (AUF-1) in myocardial cells. Therefore, we investigated if the LPS induces these RNA-binding proteins to regulate TLR4 mRNAs of cardiomyocytes, and whether the E2/ERα reduces the TLR4 mRNA stability induced by LPS through the inhibition of RNA-binding protein expression. Using a doxycycline (Dox)-induced Tet-On ERα H9c2 myocardic cell model, we also aimed to identify whether E2 and/or ERα regulate LPS-induced TLR4 mRNA stability. The results of Western blotting and reverse transcription-PCR assays demonstrated that LPS significantly increased the level of cytoplasmic HuR protein and the stability of TLR4 mRNA, and farther induced TLR4 protein expression in H9c2 cells, an effect mediated through the JNK pathway. Interestingly, E2 and/or ERα decreased the cytoplasmic HuR protein level and TLR4 mRNA stability, and farther decreased the level of TLR4 protein induced by LPS in H9c2 cardiomyoblast cells. Therefore, LPS triggered HuR expression which led to enhanced TLR4 mRNA and upregulated TLR4 expression through JNK1/2 in myocardial cells.
    Relation: CHINESE JOURNAL OF PHYSIOLOGY,57(1):8-18.
    Appears in Collections:[國際企業學系] 期刊論文

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