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http://asiair.asia.edu.tw/ir/handle/310904400/79923
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| Title: | Antiviral activity of aloe-emodin against influenza A virus via galectin-3 up-regulation. |
| Authors: | Li, SW;Li, SW;Yang, TC;Yang, TC;Lai, CC;Lai, CC;黃素華;Huang, Su-Hua;Liao, JM;Liao, JM;Wan L,;Wan L,;Lin, YJ;Lin, YJ;林振文 |
| Contributors: | 生物科技學系 |
| Keywords: | Influenza A virus;Anthraquinone;Aloe-emodin;Galectin-3 |
| Date: | 2014-05 |
| Issue Date: | 2014-07-03 15:44:40 (UTC+8) |
| Abstract: | Novel influenza A H7N9 virus, which emerged in 2013, and highly pathogenic H5N1 virus, identified since 2003, pose challenges to public health and necessitate quest for new anti-influenza compounds. Anthraquinone derivatives like aloe-emodin, emodin and chrysophanol, reportedly exhibit antiviral activity. This study probes their inhibitory mechanism and effect against influenza A virus. Of three anthraquinone derivatives, aloe-emodin, with a lower cytotoxicity showed concentration-dependently reducing virus-induced cytopathic effect and inhibiting replication of influenza A in MDCK cells. 50% inhibitory concentration value of aloe-emodin on virus yield was less than 0.05 μg/ml. Proteomics and Western blot of MDCK cells indicated aloe-emodin up-regulating galectin-3, and thioredoxin as well as down-regulating nucleoside diphosphate kinase A. Western blot and quantitative PCR confirmed aloe-emodin up-regulating galectin-3 expression; recombinant galectin-3 augmented expression of antiviral genes IFN-β, IFN-γ, PKR and 2׳,5׳–OAS in infected cells, agreeing with expression pattern of those treated with aloe-emodin. Galectin-3 also inhibited influenza A virus replication. Proteomic analysis of treated cells indicated galectin-3 up-regulation as one anti-influenza A virus action by aloe-emodin. Since galectin-3 exhibited cytokine-like regulatory actions via JAK/STAT pathways, aloe-emodin also restored NS1-inhibited STAT1-mediated antiviral responses in transfected cells: e.g., STAT1 phosphorylation of interferon (IFN) stimulation response element (ISRE)-driven promoter, RNA-dependent protein kinase (PKR) and 2׳,5׳-oligoadenylate synthetase (2׳,5׳–OAS) expression. Treatment with aloe-emodin could control influenza infection in humans. |
| Relation: | EUROPEAN JOURNAL OF PHARMACOLOGY,V.738(5):125–132. |
| Appears in Collections: | [生物科技學系] 期刊論文
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