ASIA unversity:Item 310904400/8017
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 90429/105609 (86%)
造訪人次 : 10286063      線上人數 : 112
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋


    請使用永久網址來引用或連結此文件: http://asiair.asia.edu.tw/ir/handle/310904400/8017


    題名: Molecular genetic analyses of potential beta-galactosidase genes in Xanthomonas campestris.
    作者: Yang TC;Hu RM;HsiaoYM;Weng SF;Tseng YH.
    貢獻者: Department of Biotechnology
    日期: 2003
    上傳時間: 2010-03-15 16:11:29 (UTC+8)
    出版者: Asia University
    摘要: Xanthomonas campestris pv. campestris, which displays no significant beta-1,4-D-galactopyranosidase activity, has three annotated beta-galactosidase genes in the sequenced genome, designated galA, galB and galC herein. GalA and GalB are similar to glycosyl hydrolase (GH) family 2 enzymes, including Escherichia coli LacZ. galA and galB cannot express detectable activity even after being cloned in-frame and driven by the vector's promoter. GalC is a GH35 enzyme homologous to the Xanthomonas axonopodis pv. manihotis Bga. The latter cleaves beta,1-3-linked galactose 1,000 times faster than beta,1-4-linked galactose and is not responsible for lactose utilization. In X. campestris pv. campestris cells, GalC is readily detectable by Western blotting, and the levels can be increased by cloning the gene under the control of the vector's promoter. Results of insertional mutation, transcriptional fusion assay and Western blotting indicated that galC, clustered with several GH genes, is cotranscribed with the upstream gene(s) and is expressed constitutively. Xc17L is a previously isolated mutant with elevated beta-galactosidase activity and a greatly improved ability to grow on lactose. Results of DNA sequencing of Xc17L galA, galB and galC, enzyme assays of galA, galB and galC mutants derived from Xc17L, and Western blotting of GalC in Xc17L indicated that the three beta-galactosidase genes do not encode the elevated beta-galactosidase activity in Xc17L. The presence of a fourth beta-galactosidase gene is proposed.
    關聯: The Journal of Molecular Microbiology and Biotechnology 6(3-4):145-54
    顯示於類別:[生物科技學系] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    0KbUnknown129檢視/開啟
    310904400-8017.doc35KbMicrosoft Word152檢視/開啟


    在ASIAIR中所有的資料項目都受到原著作權保護.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋