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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/8069

    Title: One-step immunochromatographic assay for the detection of Staphylococcus aureus
    Authors: Su-Hua Huang;Hsien-Chin Wei;Yeun-Chung Lee
    Contributors: Department of Biotechnology
    Keywords: Immunochromatographic assay;Staphylococcus aureus;Anti-protein A IgG;Gold nanoparticles;Nitrocellulose membrane Article Outline
    Date: 2007-08
    Issue Date: 2010-03-15 16:12:03 (UTC+8)
    Publisher: Asia University
    Abstract: An analytical system of immunochromatographic assay based on gold nanoparticles was developed to detect Staphylococcus aureus. The assay was in the sandwich format, using anti-protein A IgG with two distinct specificities. One anti-protein A IgG was immobilized in a defined detection zone on a porous nitrocellulose membrane, while the other anti-protein A IgG was conjugated with gold nanoparticles. The mixture was then passed along the porous membrane by capillary action past the anti-protein A IgG in the detection zone, binding the particles to which surface protein A was already bound to their surface, yielding a red color. The sensitivity and specificity in the immunochromatographic test were 100 and 93.0–100% for 28 S. aureus strains and 23 non-S. aureus strains, respectively. S. aureus was detected by immunochromatographic testing of two samples of precooked foods naturally contaminated with the bacterium. Eleven processed foods artificially inoculated with S. aureus at <25 CFU/g, all yielded positive results in the immunochromatographic test.
    Relation: Food Control 18(8):893-897
    Appears in Collections:[生物科技學系] 期刊論文

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