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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/8095

    Title: Genetic analysis of an attenuated Papaya ringspot virus strain applied for cross protection.
    Authors: Chiang, C.-H.;Lee, C.-Y.;Wang, C.-H.;Jan, F.-J.;Lin, S.-S.;Chen, T.-C.;Raja, J. A. J.;Yeh S.-D
    Contributors: Department of Biotechnology
    Keywords: Attenuated virus;Cross-protection;In vitro transcription;Recombinant virus
    Date: 2007
    Issue Date: 2010-03-15 16:12:19 (UTC+8)
    Publisher: Asia University
    Abstract: Papaya ringspot virus (PRSV) HA 5-1, a nitrous acid-induced mild mutant of severe strain HA, widely applied for control of PRSV by cross-protection, was used to study the genetic basis of attenuation. Using infectious clones, a series of recombinants was generated between HA 5-1 and HA and their infectivity was analyzed on the systemic host papaya and the local lesion host Chenopodium quinoa. The recombinants that contained mutations in P1 and HC-Pro genes caused attenuated infection on papaya without conspicuous symptoms, similar to HA 5-1. The recombination and sequence analyses strongly implicated two amino acid changes in the C-terminal region of P1 and two in HC-Pro of HA 5-1 involved in the attenuated infection on papaya. The recombinants that infected C. quinoa plants without local lesions contained the same mutations in the C-terminal region of HC-Pro for attenuated infection on papaya. We conclude that both P1 and HC-Pro bear important pathogenicity determinants for the infection on the systemic host papaya and that the mutations in HC-Pro affecting pathogenicity on papaya are also responsible for the inability to induce hypersensitive reaction on C. quinoa.
    Relation: European Journal of Plant Pathology 118:333-348
    Appears in Collections:[生物科技學系] 期刊論文

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