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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/8175

    Title: Effects of growth regulators on direct flowering of isolated ginseng buds in vitro, Plant Cell
    Authors: C.S. Lin;C.T. Chen;H.W. Hsiao;W.C. Chang
    Contributors: Department of Bioinformatics
    Keywords: cytokinin;direct flowering;gibberellic acid;naphthaleneacetic acid;Panax ginseng
    Date: 2005-11
    Issue Date: 2010-03-19 16:25:18 (UTC+8)
    Publisher: Asia University
    Abstract: An in vitro method for obtaining gingseng inflorescences directly from explants of gingseng (Panax ginseng) is reported. Isolated shoot-buds of somatic embryo-derived plantlets ginseng were used as explants and incubated in B5 medium supplemented with 1 mg l?1 benzyladenine (BA) and 1 mg l?1 gibberellic acid (GA3). About 15% of the buds flowered directly without developing vegetative organs. Cytokinin was found to be the key factor for inducing these isolated buds to proliferate and flower, but both these processes also occurred when benzyladenine (BA) was replaced by thidiazuron (TDZ). The optimal concentration of TDZ for obtaining the best ratios of bud proliferation and total flowering was 0.1 mg l?1, while the highest number of vegetative shoots was obtained in medium supplemented with 1 mg l?1 GA3 and 0.1 mg l?1 TDZ. The explant elongated abnormally in the presence of 10 mg l?1 GA3. Although a low concentration (1 mg l?1) of NAA increased the bud proliferation ratio in the medium supplemented with 0.1 mg l?1 TDZ and 1 mg l?1 GA3, a high concentration (5 mg l?1) of NAA reduced the bud proliferation ratio and inhibited the flowering.
    Relation: Tissue and Organ Culture 83(2): 241-244
    Appears in Collections:[生物資訊與醫學工程學系 ] 期刊論文

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