English  |  正體中文  |  简体中文  |  Items with full text/Total items : 90453/105671 (86%)
Visitors : 16016620      Online Users : 99
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version

    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/8199

    Title: Extended O-6-Methylguanine Methyltransferase Promoter Hypermethylation Following n-Butylidenephthalide Combined with 1,3-Bis(2-chloroethyl)-1-nitrosourea (BCNU) on Inhibition of Human Hepatocellular Carcinoma Cell Growth
    Authors: Yu, YL (Yu, Yung-Luen);Yu, SL (Yu, Sung-Liang);Su, KJ (Su, Kuo-Jung);Wei, CW (Wei, Chyou-Wei);Jian, MH (Jian, Min-Hui);Lin, PC (Lin, Po-Cheng);Tseng, IH (Tseng, I-Hsuan);Lin, CC (Lin, Chai-Ching);Su, CC (Su, Chin-Cheng);Chan, DC (Chan, De-Chuan);Lin, SZ (Lin, Shinn-Zong);Harn, HJ (Harn, Horng-Jyh);Chen, YLS (Chen, Yi-Lin Sophia)
    Contributors: Department of Biotechnology
    Keywords: O6-Methylguanine methyltransferase (MGMT);synergistic effect;1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU);methylation-specific PCR
    Date: 2010-10
    Issue Date: 2010-03-26 10:28:58 (UTC+8)
    Publisher: Asia University
    Abstract: Epigenetic alteration of DNA methylation plays an important role in the regulation of gene expression associated with chemosensitivity of human hepatocellular (HCC) carcinoma cells. With the aim of improving the chemotherapeutic efficacy for HCC, the effect of the naturally occurring compound n-butylidenephthalide (BP), which is isolated from a chloroform extract of Angelica sinensis, was investigated. In both HepG2 and J5 HCC cell lines, a synergistic antiproliferative effect was observed when a low dosage of BP was combined with the chemotherapeutic drug 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU). BCNU is an alkylating agent, and it prompts us to examine one of DNA repair genes, O-6-methylguanine methyltransferase (MGMT). It was evident from methylation-specific polymerase chain reaction (PCR) analysis that BP/BCNU combined treatment caused a time- and concentration-dependent enhancement of MGMT promoter methylation. Overexpression of MGMT could abolish BP-induced growth inhibition in the J5 tumor cell line as measured by colony formation assay. When BP was combined with BCNU and administered, it showed significant antitumor effects in both HepG2 and J5 xenograft tumors as compared with the use of only one of these drugs. The BCNU-induced apoptosis and inhibited MGMT protein expression in HCC cells, both in vitro and in vivo, resulting from the combination treatment of BP and BCNU suggest a potential clinical use of this compound for improving the prognosis for HCCs.
    Appears in Collections:[生物科技學系] 期刊論文

    Files in This Item:

    File Description SizeFormat
    4.doc45KbMicrosoft Word1317View/Open

    All items in ASIAIR are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback