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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/8233

    Title: Novel Quinolone CHM-1 Induces Apoptosis and Inhibits Metastasis in a Human Osterogenic Sarcoma Cell Line
    Authors: Hsu, SC (Hsu, Shu-Chun);Yang, JS (Yang, Jai-Sing);Kuo, CL (Kuo, Chao-Lin);Lo, C (Lo, Chyi);Lin, JP (Lin, Jing-Pin);Hsia, TC (Hsia, Te-Chun);Lin, JJ (Lin, Jen-Jyh);Lai, KC (Lai, Kuang-Chi);Kuo, HM (Kuo, Hsiu-Maan);Huang, LJ (Huang, Li-Jiau);Kuo, SC (Kuo, Sheng-Chu);Wood, WG (Wood, W. Gibson);Chung, JG (Chung, Jing-Gung)
    Contributors: Department of Biotechnology
    Keywords: CHM-1;human osterogenic sarcoma U-2 OS cells;apoptosis;caspase cascade;antimetastasis
    Date: 2009-12
    Issue Date: 2010-03-26 10:29:13 (UTC+8)
    Publisher: Asia University
    Abstract: Novel 2-phenyl-4-quinolone compounds have potent cytotoxic effects on different human cancer cell lines. In this study, we examined anticancer activity and mechanisms of 20-fluoro-6,7-methylenedioxy-2-phenyl-4-quinol one (CHM-1) in human osterogenic sarcoma U-2 OS cells. CHM-1-induced apoptosis was determined by flow cytometric analysis, DAPI staining, Comet assay, and caspase inhibitors. CHM-1-inhibited cell migration and invasion was assessed by a wound healing assay, gelatin zymography, and a Transwell assay. The mechanisms of CHM-1 effects on apoptosis and metastasis signaling pathways were studied using Western blotting and gene expression. CHM-1 induced G2/M arrest and apoptosis at an IC50 (3 mu M) in U-2 OS cells and caspase-3, -8, and -9 were activated. Caspase inhibitors increased cell viability after exposure to CHM-1. CHM-1-induced apoptosis was associated with enhanced ROS generation, DNA damage, decreased Delta Psi(m), levels, and promotion of mitochondrial cytochrome c release. CHM-1 stimulated mRNA expression of caspase-3, -8, and -9, AIF, and Endo G. In addition, CHM-1 inhibited cell metastasis at a low concentration (<3 mu M). CHM-1. inhibited the cell metastasis through the inhibition of MMP-2, -7, and -9. CHM-1 also decreased the levels of MAPK signaling pathways before leading to the inhibition of MMPs. In summary, CHM-1 is a potent inducer of apoptosis, which plays a role in the anticancer activity of CHM-1. (C) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27:1.637-1644, 2009
    Relation: JOURNAL OF ORTHOPAEDIC RESEARCH 27 (12): 1637-1644
    Appears in Collections:[生物科技學系] 期刊論文

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