English  |  正體中文  |  简体中文  |  Items with full text/Total items : 90429/105609 (86%)
Visitors : 10330740      Online Users : 394
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version


    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/8261


    Title: Involvement of Matrix Metalloproteinases in the Inhibition of Cell Invasion and Migration Through the Inhibition of NF-kappa B by the New Synthesized Ethyl 2-[N-p-chlorobenzyl-(2'-methyl)]anilino-4-oxo-4,5-dihydrofuran-3-carboxylate (JOTO1007) in Human Cervical Cancer Ca Ski Cells
    Authors: Huang, AC (Huang, An-Cheng);Hsu, SC (Hsu, Shu-Chun);Kuo, CL (Kuo, Chao-Lin);Liao, CL (Liao, Ching-Lung);Lai, KC (Lai, Kuang-Chi);Lin, TP (Lin, Tsung-Ping);Wu, SH (Wu, Shin-Hwar);Lu, HF (Lu, Hsu-Feng);Tang, NY (Tang, Nou-Ying);Yang, JS (Yang, Jai-Sing);Chung, JG (Chung, Jing-Gung)
    Contributors: Department of Biotechnology
    Keywords: JOTO1007;invasion;migration;MMPs;Ca Ski cells;CARCINOMA CELLS;GROWTH;APOPTOSIS;DYNAMICS;MOTILITY;PATHWAY
    Date: 2009-07
    Issue Date: 2010-03-26 10:29:25 (UTC+8)
    Publisher: Asia University
    Abstract: JOTO1007 (ethyl 2-[N-p-chlorobenzyl-(2'-methyl)] anilino-4-oxo-4,5-dihydrofuran-3-carboxylate) has anticancer effects in human cervical cancer Ca Ski cells. However, its mechanism of action on the cell migration and invasion of human cervical cancer Ca Ski cells is not fully understood. In this study, firstly, the effects of JOTO1007 on the migration and invasion of Ca Ski cells were examined by using matrigel counting. The results showed that JOT01007 suppressed the migration and invasion of the Ca Ski cells. Secondly, the effect of JOTO1007 on the levels of proteins associated with cell metastasis was examined using Western blotting. The results indicated that JOTO1007 inhibited the levels of son of sevenless homolog 1 (SOS-1), growth factor receptor-bound protein 2 (GRB2), Ras homolog gene family, member A (RhoA), Rho-associated, coiled-coil containing protein kinase 1 (ROCK-1), focal adhesion kinase (FAK), phosphorylated-c-jun (p-c-jun), nuclear factor kappa B (NF-kappa B) p65, cyclooxygenase-2 (COX-2), extracellular signal-regulated kinases 1/2 (ERK1/2), matrix metalloproteinase-2 (MMP-2), MMP-7 and MMP-9, but promoted the levels of protein kinase C (PKC), phosphoinositide 3-kinases (PI3K), MAP kinase kinase kinase 3 (MEKK-3), mitogen-activated protein kinase kinase 7 (MKK7), c-jun and inducible nitric oxide synthases (iNOS), while not affecting Ras, phosphorylated-ERK (p-ERK), p38 and c-jun N-terminal kinase 1/2 (JNK1/2), which finally led to the inhibition of migration and invasion of the Ca Ski cells in vitro. Overall, JOTO1007 inhibited NF-kappa B which then led to the inhibition of the MMP-2, -7 and -9 expression followed by the inhibition of migration and invasion in the Ca Ski cells.
    Relation: IN VIVO 23 (4): 613-619
    Appears in Collections:[生物科技學系] 期刊論文

    Files in This Item:

    File Description SizeFormat
    156.doc38KbMicrosoft Word189View/Open
    index.html0KbHTML199View/Open


    All items in ASIAIR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback