Curcumin induces apoptosis through FAS and FADD, in caspase-3-dependent and -independent pathways in the N18 mouse-rat hybrid retina ganglion cells

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Title:	Curcumin induces apoptosis through FAS and FADD, in caspase-3-dependent and -independent pathways in the N18 mouse-rat hybrid retina ganglion cells
Authors:	Lu, HF (Lu, Hsu-Feng);Lai, KC (Lai, Kuang-Chi);Hsu, SC (Hsu, Shu-Chun);Lin, HJ (Lin, Hui-Ju);Yang, MD (Yang, Mei-Due);Chen, YL (Chen, Yuan-Liang);Fan, MJ (Fan, Ming-Jen);Yang, JS (Yang, Jai-Sing);Cheng, PY (Cheng, Pi-Yun);Kuo, CL (Kuo, Chao-Lin);Chung, JG (Chung, Jing-Gung)
Contributors:	Department of Biotechnology
Keywords:	curcumin;cell cycle arrest;apoptosis;caspase;mitochondria;mouse-rat hybrid retina ganglion N18 cells;ENDOPLASMIC-RETICULUM STRESS;CANCER-CELLS;C-JUN;SARCOPLASMIC-RETICULUM;MITOCHONDRIAL PATHWAY;INDUCED EXPRESSION;LEUKEMIA-CELLS;CROSS-LINKS;DNA;ACTIVATION
Date:	2009-07
Issue Date:	2010-03-26 10:29:26 (UTC+8)
Publisher:	Asia University
Abstract:	Curcumin, a naturally occurring yellow pigment isolated from turmeric, is a well known antioxidant with broad spectrum of anti-tumor activities against many human cancer cells. In this study, curcumin-induced cytotoxic effect of mouse-rat hybrid retina ganglion cells (N18) were investigated. For determining cell viability, the trypan blue exclusion and flow cytometric analysis were used. The curcumin-caused cell cycle arrest in N18 cells was examined by flow cytometry. Curcumin affect on the production of reactive oxygen species and Ca2+ and on the decrease of the level of mitochondria membrane potential (Delta Psi(m)) were also examined by flow cytometry. Curcumin-induced apoptosis was determined by DAPI staining and Western blotting was used for examining the apoptotic signaling proteins. Cell cycle analysis showed that G2/M phase arrest and sub-G1 occurs in N18 cells following 48 h exposure to curcumin. Curcumin also caused a marked increase in apoptosis, as characterized by DNA fragmentation (sub-G1 phase formation) and DAPI staining, and dysfunction of mitochondria, which was associated with the activation of caspase-8, -9 and -3. Curcumin also promoted the levels of Fas and FADD, Bax, cytochrome c release, but decreased the levels of Bcl-2 causing changes of Delta Psi(m). Curcumin also induced endoplasmic reticulum stress in N18 cells which was based on the changes of GADD153 and GRP78 and caused Ca2+ release. Curcumin induced apoptosis through the intrinsic pathway and caspase-3-dependent and -independent pathways in N18 cells.
Relation:	ONCOLOGY REPORTS 22 (1): 97-104
Appears in Collections:	[Department of Biotechnology] Journal Article

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