Curcumin-Induced DNA Damage and Inhibited DNA Repair Genes Expressions in Mouse-Rat Hybrid Retina Ganglion Cells (N18)

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/8266

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Title:	Curcumin-Induced DNA Damage and Inhibited DNA Repair Genes Expressions in Mouse-Rat Hybrid Retina Ganglion Cells (N18)
Authors:	Lu, HF (Lu, Hsu-Feng);Yang, JS (Yang, Jai-Sing);Lai, KC (Lai, Kuang-Chi);Hsu, SC (Hsu, Shu-Chun);Hsueh, SC (Hsueh, Shu-Ching);Chen, YL (Chen, Yuan-Liang);Chiang, JH (Chiang, Jo-Hua);Lu, CC (Lu, Chi-Cheng);Lo, C (Lo, Chyi);Yang, MD (Yang, Mei-Due);Chung, JG (Chung, Jing-Gung)
Contributors:	Department of Biotechnology
Keywords:	Curcumin;DNA damage;DNA repair;N18 mouse-rat hybrid retina ganglion cells;HUMAN BREAST-CANCER;INDUCED APOPTOSIS;GEL-ELECTROPHORESIS;LIPID-PEROXIDATION;ALZHEIMERS-DISEASE;CRUDE EXTRACTS;PATHWAY;NUCLEAR;MITOCHONDRIAL;CYTOTOXICITY
Date:	2009-08
Issue Date:	2010-03-26 10:29:26 (UTC+8)
Publisher:	Asia University
Abstract:	Curcumin is reported to be a potent inhibitor of the initiation and promotion of many cancer cells. We investigated to examine whether or not curcumin induce DNA damage in mouse-rat hybrid retina ganglion cell line N18 cells. The Comet assay showed that incubation of N18 cells with 10, 25 and 30 mu M of curcumin led to a longer DNA migration smear (Comet tail). The DNA gel electrophoresis showed that 20 mu M of curcumin for 24 and 48 h treatment induced DNA damage and fragments in N18 cells. The real time PCR analysis showed that 20 mu M of curcumin for 48 h treatment decreased ATM, ATR, BRCA1, 14-3-3 sigma, DNA-PK and MGMT mRNA, and ATM and MGMT mRNA expression were inhibited in a time-dependent manner. Our results indicate that curcumin caused DNA damage and inhibited DNA repair genes which may be the factors for curcumin-inhibited cell growth.
Relation:	NEUROCHEMICAL RESEARCH 34 (8): 1491-1497
Appears in Collections:	[生物科技學系] 期刊論文

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