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    题名: Proliferation- and migration-enhancing effects of ginseng and ginsenoside Rg1 through IGF-I- and FGF-2-signaling pathways on RSC96 Schwann cells
    作者: Lu, MC;Lu, Ming-Chin;Lai, TY;Lai, Tung-Yuan;Hwang, JM;Hwang, Jin-Ming;Chen, HT;Chen, Hsien-Te;Chang, SH;Chang, Sheng-Huang;Tsai, FJ;Tsai, Fuu-Jen;Wang, HL;Wang, Hwai-Lee;Lin, CC;Lin, Chien-Chung;Kuo, WW;Kuo, Wei-Wen;Huang, CY;Huang, Chih-Yang
    贡献者: 保健系
    关键词: ginseng;Rg1;proliferate and migrate effect;Schwann cells;peripheral nerve regeneration;FIBROBLAST-GROWTH-FACTOR;NERVE REGENERATION;RATS;NEURONS;SYSTEM;HYPERTROPHY;EXPRESSION;SURVIVAL;KINASE;ROOT
    日期: 2009-06
    上传时间: 2010-03-26 10:46:21 (UTC+8)
    出版者: Asia University
    摘要: The aim of the present study is to evaluate the proliferation- and migration-enhancing effects of ginseng and its component, ginsenoside (Rg1) oil RSC96 Schwann cells. We investigated the molecular signaling pathways, which include: (1) survival signaling, IGFs-IGFIR-Akt-Bcl2 and proliferative signaling, cell cycle factors and mitogen-activated protein kinase (MAPK) pathways, (2) migrating and anti-scar signaling. FGF-2-uPA-MMPs. We treated RSC96 cells with different concentrations (100, 200, 300, 400, 500 mu g ml(-1)) of ginseng and its constituent, Rg1 (5, 10. 15, 20, 25 mu g ml(-1)). We observed a proliferative effect in a dose-dependent manner by PCNA western blotting assay, MTT assay, and wound healing test. Furthermore, we also found in the results of western blotting assay, ginseng and Rg1 enhance protein expression of IGF-I pathway regulators, cell cycle controlling proteins, and MAPK signaling pathways to promote the cell proliferation. In addition, ginseng and Rg1 also stimulated the FGF-2-uPA-MMP 9 migrating pathway to enhance the migration of RSC96 Schwann cells. Using MAPK chemical inhibitors, U0126, SB203580, and SP600125, the proliferative effects of ginseng and Rg1 on RSC96 cells were identified to be MAPK signaling-dependent. On the basis of the results, applying appropriate doses of ginseng and Rg1 with biomedical materials would be a potential approach for enhancing neuron regeneration. Copyright (C) 2009 John Wiley & Sons. Ltd.
    關聯: CELL BIOCHEMISTRY AND FUNCTION, 27(4):186-192.
    显示于类别:[食品營養與保健生技學系] 期刊論文


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