ASIA unversity:Item 310904400/86885
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 90069/105176 (86%)
造访人次 : 6421049      在线人数 : 542
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻


    题名: Chrysophanol-Induced Cell Death (Necrosis) in Human Lung Cancer A549 Cells Is Mediated Through Increasing Reactive Oxygen Species and Decreasing the Level of Mitochondrial Membrane Potential
    作者: Chien-Hang, N;Ni, Chien-Hang;Yu, Chun-Shu;Yu, Chun-Shu;Hsu-Feng Lu,;Hsu-Feng Lu,;Yan, Jai-Sing;Yang, Jai-Sing;Hua, Hui-Ying;Huang, Hui-Ying;Chen, Po-Yuan;Po-Yuan Chen,;Wu, Shin-Hwar;Wu, Shin-Hwar;Siu-Wan Ip,;Siu-Wan Ip,;鍾景光*
    贡献者: 生物科技學系
    关键词: chrysophanol;human lung cancer A549 cells;mitochondria membrane potential;necrosis;reactive oxygen species
    日期: 2014-05
    上传时间: 2014-11-07 14:46:07 (UTC+8)
    摘要: Chrysophanol (1,8-dihydroxy-3-methylanthraquinone) is one of the anthraquinone compounds, and it has been shown to induce cell death in different types of cancer cells. The effects of chrysophanol on human lung cancer cell death have not been well studied. The purpose of this study is to examine chrysophanol-induced cytotoxic effects and also to investigate such influences that involved apoptosis or necrosis in A549 human lung cancer cells in vitro. Our results indicated that chrysophanol decreased the viable A549 cells in a dose- and time-dependent manner. Chrysophanol also promoted the release of reactive oxygen species (ROS) and Ca(2+) and decreased the levels of mitochondria membrane potential (ΔΨm ) and adenosine triphosphate in A549 cells. Furthermore, chrysophanol triggered DNA damage by using Comet assay and DAPI staining. Importantly, chrysophanol only stimulated the cytocheome c release, but it did not activate other apoptosis-associated protein levels including caspase-3, caspase-8, Apaf-1, and AIF. In conclusion, human lung cancer A549 cells treated with chrysophanol exhibited a cellular pattern associated with necrotic cell death and not apoptosis in vitro. © 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 740-749, 2014.
    Copyright © 2012 Wiley Periodicals, Inc., a Wiley company.
    显示于类别:[生物科技學系] 期刊論文




    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈