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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/86928


    Title: Protective effect of Danggui (Radix Angelicae Sinensis) on angiotensin II-induced apoptosis in H9c2 cardiomyoblast cells
    Authors: 黃志揚;Huang, Chih-yang;Kuo, Wei-Wen;Kuo, Wei-Wen;Kuo, Chia-Hua;Kuo, Chia-Hua;蔡輔仁;Liu, Peng-Yu;Liu, Peng-Yu;Dennis, Jine-;Hsieh, Dennis Jine-Yuan;*
    Contributors: 生物科技學系
    Keywords: Traditional Chinese Medicine (TCM);Danggui;Radix Angelicae Sinensis;Angiotensin II;Cardiomyoblast;Apoptosis
    Date: 2014
    Issue Date: 2014-11-07 14:52:12 (UTC+8)
    Abstract: Background
    Danggui (Radix Angelicae Sinensis) is an herb often used in Traditional Chinese medicine. It is used to promote blood flow and has been used in the treatment of myocardial ischemia-reperfusion injury in animal models. Angiotensin II (Ang II) has been shown to play important roles in mediating cardiovascular diseases, and may cause cardiac hypertrophy and apoptosis. This study aimed to investigate whether Danggui has protective effects on Ang II-induced apoptosis in H9c2 cardiomyoblast cells and study the mechanisms involved.

    Methods
    We evaluated the effect of Danggui on Ang II-induced apoptosis in an in vitro model. H9c2 cardiomyoblast cells were cultured in serum-free medium for 4 hr, then treated with Danggui (50, 100 μg/ml) 1 hr pre- or post-Ang II treatment. After a further 23 hr of culture, cells were harvested for analyses with assays for apoptosis markers and cell signaling pathways.

    Results
    Our results showed that Ang II induced upregulation of pro-apoptotic Bad, instability of the mitochondria membrane potential, cytochrome c release, caspase-9 and caspase-3 activation and cardiomyocyte apoptosis. Pre- or post-treatment with Danggui reversed all of the above Ang II-induced apoptotic effects in H9c2 cells. Furthermore, the JNK (SP600125) inhibitor completely blocked Danggui inhibition of caspase-3 activation in Ang II-treated H9c2 cells.

    Conclusions
    Our results showed that Danggui either pre-treatment or post-treatment highly attenuated the Ang II-induced apoptosis in cardiomyoblast cells. The findings demonstrated that the anti-apoptosis effect of Danggui is mediated by JNK and PI3k inhibitors.
    Relation: BMC Complementary and Alternative Medicine
    Appears in Collections:[生物科技學系] 期刊論文

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