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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/87117

    Title: Prenatal diagnosis and molecular cytogenetic characterization of chromosome 22q11.2 deletion syndrome associated with congenital heart defects
    Authors: Yu-Ling Kuo;Chih-Ping Chen;Liang-Kai Wang;Tsang-Ming Ko;Tung-Yao Chang;Schu-Rern Chern;Peih-Shan Wu;Yu-Ting Chen;Shu-Yuan Chang
    Contributors: 生物科技學系
    Keywords: 22q11.2 deletion syndrome;congenital heart defects;prenatal diagnosis
    Date: 2014-06
    Issue Date: 2014-12-16 17:01:22 (UTC+8)
    Abstract: Objective
    To report prenatal diagnosis of 22q11.2 deletion syndrome in a pregnancy with congenital heart defects in the fetus.

    Case report
    A 26-year-old, primigravid woman was referred for counseling at 24 weeks of gestation because of abnormal ultrasound findings of fetal congenital heart defects. The Level II ultrasound revealed a singleton fetus with heart defects including overriding aorta, small pulmonary artery, and ventricular septal defect. Cordocentesis was performed. The DNA extracted from the cord blood was analyzed by multiplex ligation-dependent amplification (MLPA). The MLPA showed deletion in the DiGeorge syndrome (DGS) critical region of chromosome 22 low copy number repeat (LCR) 22-A∼C. Conventional cytogenetic analysis revealed a normal male karyotype. Repeated amniocentesis and cordocentesis were performed. Whole-genome array comparative genomic hybridization (aCGH) on cord blood was performed. aCGH detected a 3.07-Mb deletion at 22q11.21. Conventional cytogenetic analysis of cultured amniocytes revealed a karyotype 46,XY. Metaphase fluorescence in situ hybridization (FISH) analysis on cultured amniocytes confirmed an interstitial 22q11.2 deletion.

    Prenatal ultrasound findings of congenital heart defects indicate that the fetuses are at increased risk for chromosome abnormalities. Studies for 22q11.2 deletion syndrome should be considered adjunct to conventional karyotyping. Although FISH has become a standard procedure for diagnosis of 22q11.2 deletion syndrome, MLPA can potentially diagnose a broader spectrum of abnormalities, and aCGH analysis has the advantage of refining the 22q11.2 deletion breakpoints and detecting uncharacterized chromosome rearrangements or genomic imbalances.
    Relation: Taiwanese Journal of Obstetrics & Gynecology,53(2),248–251.
    Appears in Collections:[生物科技學系] 期刊論文

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