ASIA unversity:Item 310904400/87122
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    题名: PAX3 Loads onto Pericentromeric Heterochromatin During S Phase Through PARP1
    作者: 吳祖方;TSU-FANG, WU;姚雅莉;Yao, Ya-Li;賴羿如;LAI, I-LU;李宗翰;LEE, TSUNG-HAN;UNDE, D.ALAN;UNDERHILL, D.ALAN;楊文明;YANG, WEN-MING
    贡献者: 生物科技學系
    关键词: PARP1;PAX3;S phase;pericentromeric heterochromatin
    日期: 2014-07
    上传时间: 2014-12-16 17:02:23 (UTC+8)
    摘要: BACKGROUND:
    Proper re-establishment of heterochromatin after each round of DNA replication is critical to the preservation of cell identity. Paired box 3 (PAX3), a transcription factor important in embryonic development, was found to mediate the formation of pericentromeric heterochromatin. However, how PAX3 recognizes the heterochromatic environment and re-establishes it after DNA replication remains unclear.
    MATERIALS AND METHODS:
    Cell-cycle synchronization, fluorescence microscopic analyses, and co-immunoprecipitation were used to analyze the heterochromatic localization of PAX3 in HEK 293 cells and NIH 3T3 cells.
    RESULTS:
    We found that PAX3 binds pericentromeric heterochromatin during middle-to-late S phase. Loading of PAX3 onto pericentromeric heterochromatin requires poly(ADP-ribose) polymerase 1 (PARP1). Furthermore, loss of PAX3 or PARP1 delays cell-cycle progression through the S phase.
    CONCLUSION:
    Our results reveal how PAX3 recognizes and maintains pericentromeric heterochromatin at the S phase of the cell cycle.
    Copyright© 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.
    關聯: ANTICANCER RESEARCH;34(9):4717-22.
    显示于类别:[生物科技學系] 期刊論文

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