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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/87175


    Title: Earthworm;Pheretima aspergillum) extract stimulates osteoblast activity and inhibits osteoclast differentiation
    Authors: Yuan-Tsung, F;Fu, Yuan-Tsung;Chen, Kuo-Yu;Chen, Kuo-Yu;Yueh-Sheng, C;Chen, Yueh-Sheng;姚俊旭;Yao, Chun-Hsu;*
    Contributors: 生物與醫學資訊學系
    Keywords: Earthworm;Osteoblasts;Osteoclasts
    Date: 2014-12
    Issue Date: 2014-12-30 11:22:51 (UTC+8)
    Abstract: Background
    The potential benefits of earthworm (Pheretima aspergillum) for healing have received considerable attention recently. Osteoblast and osteoclast activities are very important in bone remodeling, which is crucial to repair bone injuries. This study investigated the effects of earthworm extract on bone cell activities.

    Methods
    Osteoblast-like MG-63 cells and RAW 264.7 macrophage cells were used for identifying the cellular effects of different concentrations of earthworm extract on osteoblasts and osteoclasts, respectively. The optimal concentration of earthworm extract was determined by mitochondrial colorimetric assay, alkaline phosphatase activity, matrix calcium deposition, Western blotting and tartrate-resistant acid phosphatase activity.

    Results
    Earthworm extract had a dose-dependent effect on bone cell activities. The most effective concentration of earthworm extract was 3 mg/ml, significantly increasing osteoblast proliferation and differentiation, matrix calcium deposition and the expression levels of alkaline phosphatase, osteopontin and osteocalcin. Conversely, 3 mg/ml earthworm extract significantly reduced the tartrate-resistant acid phosphatase activity of osteoclasts without altering cell viability.

    Conclusions
    Earthworm extract has beneficial effects on bone cell cultures, indicating that earthworm extract is a potential agent for use in bone regeneration.
    Relation: BMC Complementary and Alternative Medicine
    Appears in Collections:[生物資訊與醫學工程學系 ] 期刊論文

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