| Abstract: | Doxorubicin is a widely chemotherapeutic drug for treat-ing cancer, but doxorubicin is dose dependent to damage heart cause cardiac toxicity. Q10 might effectively reduce cardiotoxicity caused by doxorubicin, to protect the function of the heart, often used in the medical nutritional supplements on cancer treatment. This study focuses on previously used Q10 whether can achieve the prevention of cardiovascular disease caused by doxorubicin treatment.
4 weeks SD rats adapt lab for 4 weeks as animal model and randomly divided into control, doxorubicin, oral Q10 treatment by doxorubicin (Dox+Q10) and Q10 four groups, control group is oral water 10 ml/kg every day and IP physio-logical saline, doxorubicin groups is IP doxorubicin 2.5 mg/kg every three days and Q10 groups is oral Q10 10 mg/kg every day, all groups treat 3 weeks and then animal sacrifice, analysis heart weight by one way ANOVA statistics and western blot analysis TGF β1, CTGF, COL1A1, MMP-9 and MMP-2 in fibrosis pathway, Bak, Bax, cytochrome c, caspase-9 and caspase-3 in apoptosis and p-PI3K, p-Akt, p-Bad, Bcl-2 and Bcl-xL in survival by mitochondria dependent pathway, Fas-L, FADD and caspase-8 in cell death pathway. Found heart of doxorubicin damage caused decreased heart weight, cardiomyocytes arrangement disarray and numerous of collagen accumulation, enhanced fibrosis pathway and cell death pathway, inhibited survival protein to cause cardiomyocytes apoptosis. However, advance use Q10 then damaged by doxorubicin, Q10 can protect cardiomyocytes and reduce fibrosis pathway and cell death pathway protein expression, enhance sruvival protein expression and inhibit cardiomyocytes apoptosis. Therefore, Q10 can indeed found to prevent heart damage caused by doxorubicin achieve a protective effect. |
