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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/9415


    Title: 食品安全性研究-河魨製品相關之基因定性定量研究與毒素之基因毒理探討
    Authors: 謝承紘
    Contributors: 健康學院
    保健營養生技學系
    Keywords: 河魨、
    基因條碼、
    基因掌紋圖譜、
    基因毒理
    即時定量PCR
    河魨毒生產菌
    Puffer fish
    DNA barcode
    real time quantitative PCR
    TTX-producing bacteria,
    gene fingerprint
    genetic toxicity
    Date: 2009
    Issue Date: 2010-05-07 11:23:24 (UTC+8)
    Abstract: “毒奶粉”事件凸顯了食品衛生安全的重要性。申請者主要研究對象為含河魨毒、熱
    帶性海魚毒或箱魨毒之魨科魚類,此類魚種之誤食或濫用,極可能產生嚴重致死中毒
    事件,其重要性更不在話下。本計畫擬延續先前計畫所得豐富基因圖譜資訊及加工品
    基因條碼分析系統,擴大深入探討下列三大方向:1. 不等比例混合魨科加工製品之微
    量基因萃取法建立及real-time PCR 定量分析。2. 外因性河魨毒生產來源追溯及產毒微
    生物基因體圖譜分析。3. 利用微生物量產河魨毒之最適條件探討及河魨毒之基因毒理
    影響。期望建立下列幾點成果:1. 建立當食品中摻雜魨科魚肉時,可快速萃取出微量
    且裂解之殘存DNA 的方法,並利用即時定量PCR 迅速分析產品中所含之比例及偵測
    極限,杜絕日後可能產生之摻假或假偽高價魚類之製品的販售,可能中毒之食用安全
    性顧慮的預防,並提供政府擬定相關漁業政策。2. 瞭解台灣地區區域性河魨毒生產菌
    之菌種,在學術上可建立該種河魨毒生產菌之基因圖譜掌紋資料,明瞭河魨毒生產菌
    之間基因體的同質性,與其他菌種間之差異性,更可提供國內外相關研究之參考依據。
    3. 建立微生物量產河魨毒模式,在學術上可建立河魨毒與基因之間所產生之毒理影
    響,提供國內外醫學界研發麻醉藥或止痛藥,甚至河魨毒抗體疫苗的重要依據。
    The "poisonous milk power" incident demonstrated the importance of food hygiene. The
    research samples of our project are puffer fish species which might contain tetrodotoxin,
    ciguatoxin, and ostracitoxin/Pahutoxin. Abused or mistaked eating of these kinds of puffer
    fish will produce the poisoning of death. Therefore, it is more important than previous
    mentioned incident. This project will continuously research following the established gene
    fingerprint and DNA barcode systems from the previous program and deeply extend to study
    the following three directions: 1. Studies on the method of DNA extraction from the mixed
    meat with puffer fish and quantitative analysis by real time PCR. 2. Studies on the exogenous
    of tetrodotoxin-producing bacteria and analysis their genome fingerprint. 3. Studies on the
    method of high amount TTX extraction from TTX-producing bacteria with the optimum
    growth condition and the mechanism of genetic toxicity between TTX with nucleobase. The
    expectation of this project wish to com to the following achievements: 1. Wish to establish the
    optimum method for degraded DNA extraction of the mixed meats from commercial fish
    products based on the puffer fish. Quantitative analysis with these commercial products by
    using real time PCR method and detection limit will also be established in order to stop the
    adulteration and misused of puffer fish. These results will be the base for preventing food
    poisoning and improve the government to ban or draft relevant fishery's policies for the
    purpose of food control. 2. Understand the exogenous of regional tetrodotoxin-producing
    bacteria of Taiwan and establish the gene fingerprints of these TTX-producing bacteria. The
    homogeneity and heterogeneity of genomes from the TTX-producing bacteria and other
    bacteria are the more important data and even more can offer as the basic reference for the
    domestic and international relevant further research on academy. 3. Establish the method of
    high amount TTX extraction from TTX-producing bacteria with the optimum growth
    condition and understand the mechanism of genetic toxicity between TTX with nucleobase.
    Moreover, these data can offer the further medical research to develop narcotic drug,
    analgesic, and the antibody vaccine of TTX.
    Appears in Collections:[食品營養與保健生技學系] 科技部研究計畫

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