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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/9435


    Title: 新穎奈米微粒載體於抗癌藥物傳遞的研究
    Authors: 蔡政芳
    Contributors: 健康學院
    生物科技學系
    Keywords: 幾丁聚醣
    奈米粒子
    藥物制放
    chitosan
    controlled drug delivery system
    nanoparticles
    Date: 2008
    Issue Date: 2010-05-07 14:07:49 (UTC+8)
    Abstract: 奈米藥物輸遞系統可藉由改變藥物在人體內的分佈,達到高度靶向、控制藥物釋放、提高難溶藥物的溶解率及吸收率,增加藥效及減少藥物毒性等目的。本研究以聚麩胺酸或三聚磷酸鈉 (sodium tripolyphosphate) 與小分子量幾丁聚醣經由離子凝膠法(ionic gelation process)製備奈米粒子,作為抗癌藥物傳遞載體。比較由不同去乙醯度、分子量的幾丁聚醣所製成奈米粒子特性的差異,並觀察其對抗癌藥物Doxorubicin 與Paclitaxel 包覆率及體外釋放速率的影響。
    本計畫擬於三年內完成。第一年主要對所製備的奈米粒子作粒徑分析、表面電荷分析與外觀型態的觀察等,進而找出形成奈米粒子的最佳條件。第二年是以Caco-2 細胞作為體外試驗模式,評估幾丁聚醣奈米粒子,對於細胞的毒性與穿透能力。並於37℃時模擬人體腸道 (pH 7.4)、胃道 (pH 1.2) 做體外釋放。第三年探討幾丁聚醣奈米粒子載負高疏水性藥物紫杉醇。探討包覆效率、藥物含量、藥物釋放及對卵巢癌細胞毒性測試。

    Nanoscale drug delivery system has been developed to convey a sufficient dose of drug to lesion, provide drug targeting, increase the efficiency of drug delivery and improve release profiles. The objectives of this study is to evaluate potential of chitosan nanoparticles as carriers for the anticancer drug. This novel delivery system is prepared using a simple and mild ionic- gelation method to which a poly-γ-glutamic acid (γ-PGA) or tripolyphosphate pentasodium (TPP) solution was added to a low molecular-weight chitosan. To investigate the effect of degree of deacetylation and molecular weight of chitosan on nanoparticles characteristics prepared by ionotropic gelation and its application on the controlled release of Doxorubicin and Paclitaxel.
    In the first year, the investigation is to evaluate the physicochemical characteristics of these prepared nanoparticles, including the analyses of particle size, surface charges and the morphology, and then finding the optimal condition for forming nanoparticles. For the second year, we will evaluate the activity of chitosan nanoparticles by studying the effect of nanoparticles on Caco-2 in vitro cell cytotoxicity and permeability. Release study was conducted by in vitro investigation to simulate intestinal fluid and gastric fluid at 37℃. Finally, in the third year, we will to expected that drug-loaded chitosan nanoparticles for hydrophobic drugs such as the most representative drug of Paclitaxel. The drug loading content and loading efficiency, and release profile of the prepared nanoparticles and their cytotoxicity on HeLa cells were investigated in vitro.
    Appears in Collections:[生物科技學系] 科技部研究計畫

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