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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/95279


    Title: Sambucus williamsii induced embryonic stem cells differentiated into neurons
    Authors: Liu, 劉詩平;Shih-Ping
    Contributors: 社會工作學系
    Date: 2015-03
    Issue Date: 2015-11-12 14:31:37 (UTC+8)
    Abstract: The pluripotent stem cells, including embryonic stem cells (ESCs), are capable of self-renewal and differentiation
    into any cell type, thus making them the focus of many clinical application studies. However, the efficiency
    of ESCs differentiated into neurons needs to improve. In this study, we tried to increase efficiently
    to a neural fate in the presence of various transitional Chinese medicines through a three-step differentiation
    strategy. From extracts of 10 transitional Chinese medicine candidates, we determined that Sambucus
    williamsii (SW) extract triggers the up-regulation of Nestin and Tuj1 (neuron cells markers) gene expression
    levels. After determining the different concentrations of SW extract, the number of neurons in the 200 μg/ml
    SW extract group was higher than the control, 50, 100, and 400 μg/ml SW extract groups. In addition, the
    number of neurons in the 200 μg/ml SW extract group was higher and higher after each time passage (three
    times). We also detected the Oct4, Sox2 (stem cells markers), Tuj1, and Nestin genes expression levels by
    RT-PCR. In the differentiated process, Oct4 and Sox2 genes decreased while the Tuj1 and Nestin genes expression
    levels increased. In summary, we demonstrated that SW could induce pluripotent stem cells differentiated
    into neurons. Thus, SW might become a powerful material for neurons–differentiating strategies.
    Relation: BioMedicine (ISSN 2211-8039)
    March 2015, Vol. 5, No. 1, Article 3;Pages 19-23
    Appears in Collections:[社會工作學系] 期刊論文

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